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Hydration test : DPM 9003 device (NOVA, USA) attached to a 4-mm sensor probe was used to measure the impedance of EFT-412 skin tissues. Briefly, after two weeks incubation in vehicle- or MB-containing medium, the EFT-412 tissue insert was removed from the 6-well plate and placed on a borrow money Milfay OK get loan sterile pad in the culture hood. The tissue surface was pat dried with sterile cotton-tipped swabs and then air-dried for additional two minutes. The impedance of four-quarter circles was measured clockwise with the average value representing the hydration of each tissue.
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